Coding
Part:BBa_K1104101:Experience
Designed by: Yin-Chih Chen, LIANG-CHUN CHEN Group: iGEM13_NYMU-Taipei (2013-10-27)
Expression check
We ligated this part with LacI regulated promoter. In order to know whether this biobrick is correctly expressed in the E. coli, we have done digestion on XbaI and PstI cutting site to proof that the length of our biobrick is right. So there is only the coding sequence of RFP-FimH instead of RFP only. The picture on the left is the result of the electrophoresis after the digestion. Secondly, through observing the fluorescence emitted by the E. coli we can be sure that we have produced the protein that we want, which is the RFP-FimH fusion protein. The result is as the picture on the right.
Applications of BBa_K1104101
User Reviews
UNIQe0b8e6e336693bd6-partinfo-00000000-QINU UNIQe0b8e6e336693bd6-partinfo-00000001-QINU