Composite

Part:BBa_K1139025

Designed by: Shinya Suzuki   Group: iGEM13_Tokyo_Tech   (2013-10-21)
Revision as of 16:57, 26 October 2013 by Fujimon (Talk | contribs)

Pbad-M13 on pSB3

M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin　and a packaging sequence. The promoter upstream of g2p is altered to bad promoter. A phage particle is formed only when the host cell receives arabinose because g2p (gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 237
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6165
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 136


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Categories
Parameters
None