Coding

Part:BBa_K1088004

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-08-06)
Revision as of 11:33, 28 October 2013 by Thigers (Talk | contribs)

IspG (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase)

1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (IspG) is part of the bacterial MEP (methylerythritol phosphate) pathway used for production of isoprenoids.

From ecocyc: "IspG catalyzes the conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. IspG activity depends on as-yet unidentified additional proteins, most likely involved in the oxidation portion of the reaction" [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10370 (Link)]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 343


[edit]
Categories
Parameters
None