Coding

Part:BBa_K1031017

Designed by: He Shuaixin   Group: iGEM13_Peking   (2013-10-22)
Revision as of 12:45, 25 October 2013 by Psyche (Talk | contribs)

J23114-B0034-RiboJ-φR73δ

For detailed information concerning Band-pass Filter, please visit 2013 Peking iGEM Band-pass Filter/a>


Characteristic

We constructed this circuit to test the inhibition effect of C1 repression circuit. It is composed of constitutive promoter J23114, Ribosome Binding Site B0034 and RiboJ enzyme.

Fig 1 Construction of J23114-φR73Δ circuit. The orange arrowhead stands for promoter J23114, the green oval represents RBS B0034, φR73Δ shown in brown square. == '''Features and Sequence''' == Sequence and Features BBa_K1031017 SequenceAndFeatures == '''Usage in Band-pass Filter''' == This circuit is an assistant device aiming at testing the inhibition effect of C1 protein to hybrid promoter. We propose to construct a high fluorescence background level, and when C1 protein is expressed, the fluorescence intensity is supposed to go down if the inhibition circuit really works.

Fig 3 Usage of J23114-φR73Δ circuit in Band-pass Filter. It is co-transformed with C1 inhibition circuit to construct high fluorescence background level.

[edit]
Categories
//cds/transcriptionalregulator/activator
//function/regulation/transcriptional
Parameters
biology