Translational_Unit

Part:BBa_K1223005

Designed by: Orr Schlesinger   Group: iGEM13_BGU_Israel   (2013-09-07)
Revision as of 12:44, 12 October 2013 by Alex m (Talk | contribs)

cI translational unit

Lambda cI translational unit with BBa_K354000 as promoter


Design notes

This part was designed to function as a biological timer for our P.A.S.E 1 system. It includes an assembly of two existing parts from the Registry, Lac/Ara-1 IPTG Inducible promoter (BBa_K354000) and LVA-tagged cI repressor protein (BBa_K327018). In order to extend the half life of the cI protein, we removed the LVA tail. The part was characterized through sequencing and restriction digest with PvuII and HindIII.


CI1.png

lane1: undigested pUC57-cI

lane2: pUC57-cI digested with PvuII and HindIII

lane3: DS5000 ladder


In addition, the promoter's performance under the presence of IPTG and Arabinose was analyzed via comassie staining. The cI protein is 29 KDa.

CI2.jpg


Source

genebank, iGEM parts registry


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None