Part:BBa_K1140006:Experience

Applications of BBa_K1140006

The team observed that this part works correctly in E. coli K12.

Figure 1. Temperature dependence of mCherry translation by u6 RNA thermometer in E. coli. Tet repressor is NOT present in this test. Two control cultures without mCherry sequence are included for growth and color comparison. a) Control 30ºC b)Control 37ºC c)30ºC d)37ºC.

Figure 2. Relative fluorescence under 25, 30, 37 and 42 celcius grades in E. coli. M1 is a group of cultures used by UANL_Mty-Mexico team. Tet repressor is NOT present in this test.

Figure 3. Behavior of different clones transformed with this construction. Relative fluorescence under 25, 30, 37 and 42 celcius grades in E. coli. M1, 2, 11 and 12. Surprisingly, we obtained different behaviors from the same DNA. All measurements were performed at least in triplicate, the aritmethic mean is shown. Figure 2 shows the behavior of our best clone, dubbed M1

Figure 4. We found that a gaussian function fits our data well, and it provides us a way to quantify the strength (amplitude), optimal value (horizontal shift), and definition or clearness (width) of our RNAT activity. We believe positive slope is due to RNAT melting, while negative slope is due to increase in the overall protein degradation rate due to higher temperatures.

User Reviews

UNIQ182b9a57b162a3a4-partinfo-00000000-QINU UNIQ182b9a57b162a3a4-partinfo-00000001-QINU