Regulatory
Part:BBa_K950002
Designed by: Jakob Matthes Group: iGEM12_Tuebingen (2012-07-29)
Revision as of 03:42, 5 October 2013 by Jakobmatthes (Talk | contribs)
yeast anb1 promotor
This is the promotor of yeast anb1 gene. Panb1 is a target of the rox1 repressor, see Part BBa_K950001
Basal expression level of mOrange under the control of the promotors Pfet3, Panb1 and Psuc2. Non-transformed yeast was used as negative control. Fluorescence was detected using a RFP filter set (ET Bandpass 470/40, ET Bandpass 572/35).
Basal expression level of mOrange under the control of the promotors Pfet3, Panb1 and Psuc2. Non-transformed yeast was used as negative control. Fluorescence was measured using a plate reader (Ex: 548nm +/-9nm, Em: 581nm +/-20nm) and normalized to OD600 of the cell suspension. All measurements were performed as triplicates.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//chassis/eukaryote/yeast
//promoter
//regulation/negative
//promoter
//regulation/negative
None |