Coding

Part:BBa_K1159000

Designed by: Louise FUNKE   Group: iGEM13_TU-Munich   (2013-05-17)
Revision as of 03:44, 5 October 2013 by IngmarPolte (Talk | contribs)

Erythromycin Esterase Type II (EreB) in RFC[25]

Erythromycin esterase type II from Escherichia coli that degrades macrolid antibiotics. Part is RFC[25] compatible and is flanked by RFC[25] pre- and suffix.

<th id="AutoAnnotatorHeader" colspan="2">Protein data table for BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_BBa_K1159000">BBa_BBa_K1159000</a> automatically created by the <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">BioBrick-AutoAnnotator</a> version 1.0</th></tr><tr><td class="AutoAnnotator1col" colspan="2">Nucleotide sequence in RFC 25, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein)
 ATGGCCGGCAGGTTCGAA ... GTTTATGAAACCGGT
 ORF from nucleotide position -8 to 1260 (excluding stop-codon)</td></tr><tr><td class="AutoAnnotator1col" colspan="2">Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)
1 
101 
201 
301 
401 		MAGRFEEWVKDKHIPFKLNHPDDNYDDFKPLRKIIGDTRVVALGENSHFIKEFFLLRHTLLRFFIEDLGFTTFAFEFGFAEGQIINNWIHGQGTDDEIGR
FLKHFYYPEELKTTFLWLREYNKAAKEKITFLGIDIPRNGGSYLPNMEIVHDFFRTADKEALHIIDDAFNIAKKIDYFSTSQAALNLHELTDSEKCRLTS
QLARVKVRLEAMAPIHIEKYGIDKYETILHYANGMIYLDYNIQAMSGFISGGGMQGDMGAKDKYMADSVLWHLKNPQSEQKVIVVAHNAHIQKTPILYDG
FLSCLPMGQRLKNAIGDDYMSLGITSYSGHTAALYPEVDTKYGFRVDNFQLQEPNEGSVEKAISGCGVTNSFVFFRNIPEDLQSIPNMIRFDSIYMKAEL
EKAFDGIFQIEKSSVSEVVYETG*
</td></tr><tr><td class="AutoAnnotator1col" colspan="2">Sequence features: (with their position in the amino acid sequence, see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software/FeatureList">list of supported features</a>)
None of the supported features appeared in the sequence
</td></tr><tr><td class="AutoAnnotator1col" colspan="2">Amino acid composition:
Ala (A)27 (6.4%)
Arg (R)16 (3.8%)
Asn (N)20 (4.7%)
Asp (D)29 (6.9%)
Cys (C)3 (0.7%)
Gln (Q)14 (3.3%)
Glu (E)30 (7.1%)
Gly (G)31 (7.3%)
His (H)15 (3.5%)
Ile (I)36 (8.5%)
Leu (L)34 (8.0%)
Lys (K)29 (6.9%)
Met (M)12 (2.8%)
Phe (F)31 (7.3%)
Pro (P)14 (3.3%)
Ser (S)22 (5.2%)
Thr (T)19 (4.5%)
Trp (W)4 (0.9%)
Tyr (Y)19 (4.5%)
Val (V)18 (4.3%)
</td></tr><tr><td class="AutoAnnotatorAminoCountingOuter">Amino acid counting
Total number:423
Positively charged (Arg+Lys):45 (10.6%)
Negatively charged (Asp+Glu):59 (13.9%)
Aromatic (Phe+His+Try+Tyr):69 (16.3%)
</td><td class="AutoAnnotatorBiochemParOuter">Biochemical parameters
Atomic composition:C2204H3348N568O636S15
Molecular mass [Da]:48459.2
Theoretical pI:5.55
Extinction coefficient at 280 nm [M-1 cm-1]:50310 / 50498 (all Cys red/ox)
</td></tr><tr><td class="AutoAnnotator1col" colspan="2">Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges <input type='button' id='hydrophobicity_charge_button' onclick='show_or_hide_plot_1380912466041()' value='Show'></td></tr><tr><td class="AutoAnnotator1col" colspan="2">Codon usage
Organism:E. coliB. subtilisS. cerevisiaeA. thalianaP. patensMammals
Codon quality (<a href="http://en.wikipedia.org/wiki/Codon_Adaptation_Index">CAI</a>):good (0.74)good (0.77)good (0.75)good (0.80)good (0.76)good (0.66)
</td></tr><tr><td class="AutoAnnotator1col" colspan="2">Alignments (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)
SwissProt:<a href='http://www.uniprot.org/uniprot/P05789'>P05789</a> (100% identity on 418 AAs), <a href='http://www.uniprot.org/uniprot/P07684'>P07684</a> (24% identity on 381 AAs)
TrEML:<a href='http://www.uniprot.org/uniprot/Q2V0Y9'>Q2V0Y9</a> (100% identity on 418 AAs), <a href='http://www.uniprot.org/uniprot/D7J5T5'>D7J5T5</a> (44% identity on 402 AAs)
PDB:<a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=2qgm'>2qgm</a> (20% identity on 373 AAs), <a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=2rad'>2rad</a> (19% identity on 386 AAs)
</td></tr><tr><th id='AutoAnnotatorHeader' colspan="2">Predictions (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)</th></tr><tr><td class="AutoAnnotatorLocalizationOuter">Subcellular Localization (reliability in brackets)
Archaea:cytosol (100%)
Bacteria:cytosol (100%)
Eukarya:cytosol (58%)
</td><td class="AutoAnnotatorGOOuter">Gene Ontology (reliability in brackets)
Molecular Function Ontology: -
Biological Process Ontology:<a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0046677'>GO:0046677</a> (51%)
 
</td></tr><tr><td class="AutoAnnotator1col" colspan="2">Predicted features:
Disulfid bridges: -
Transmembrane helices: -
</td></tr><tr><td class="AutoAnnotator1col" colspan="2"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For more information please see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">documentation</a>.
If you have any questions, comments or suggestions, please leave us a <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">comment</a>.</td></tr></table></div>
<script type='text/javascript' src='http://code.jquery.com/jquery-1.10.0.min.js'></script><script type='text/javascript' src='http://2013.igem.org/Team:TU-Munich/Flot.js?action=raw&ctype=text/js'></script><script>function show_or_hide_plot_1380912466041(){hydrophobicity_datapoints = [[2.5,0.32],[3.5,-0.76],[4.5,-1.82],[5.5,-1.92],[6.5,-0.18],[7.5,-1.52],[8.5,-1.52],[9.5,-1.60],[10.5,-2.06],[11.5,-2.00],[12.5,-1.54],[13.5,-0.28],[14.5,-0.28],[15.5,1.12],[16.5,-0.48],[17.5,-0.80],[18.5,-1.68],[19.5,-1.60],[20.5,-3.06],[21.5,-3.06],[22.5,-2.68],[23.5,-3.06],[24.5,-3.06],[25.5,-1.80],[26.5,-1.88],[27.5,-1.94],[28.5,-0.48],[29.5,-0.68],[30.5,-2.02],[31.5,-0.34],[32.5,0.88],[33.5,0.04],[34.5,0.24],[35.5,0.88],[36.5,-0.92],[37.5,-0.98],[38.5,-0.06],[39.5,1.00],[40.5,1.90],[41.5,2.72],[42.5,1.18],[43.5,-0.36],[44.5,-0.88],[45.5,-2.28],[46.5,-1.64],[47.5,-0.04],[48.5,-0.12],[49.5,-0.66],[50.5,0.54],[51.5,0.54],[52.5,0.40],[53.5,1.94],[54.5,1.74],[55.5,0.54],[56.5,-0.16],[57.5,-0.16],[58.5,-0.16],[59.5,-0.16],[60.5,1.04],[61.5,1.74],[62.5,1.88],[63.5,0.42],[64.5,0.62],[65.5,0.82],[66.5,0.18],[67.5,-0.16],[68.5,0.40],[69.5,0.96],[70.5,0.76],[71.5,1.20],[72.5,1.20],[73.5,0.64],[74.5,1.34],[75.5,0.70],[76.5,0.90],[77.5,0.70],[78.5,0.70],[79.5,0.06],[80.5,-0.56],[81.5,-0.22],[82.5,0.32],[83.5,0.32],[84.5,-0.30],[85.5,0.22],[86.5,0.22],[87.5,-1.32],[88.5,-0.70],[89.5,-0.70],[90.5,-0.60],[91.5,-1.64],[92.5,-1.70],[93.5,-2.32],[94.5,-2.32],[95.5,-1.34],[96.5,-1.28],[97.5,-1.48],[98.5,-0.22],[99.5,1.24],[100.5,-0.44],[101.5,-1.00],[102.5,0.46],[103.5,-0.36],[104.5,-1.38],[105.5,-0.92],[106.5,-0.98],[107.5,-2.24],[108.5,-1.22],[109.5,-1.74],[110.5,-1.56],[111.5,-1.00],[112.5,0.26],[113.5,0.26],[114.5,0.86],[115.5,1.76],[116.5,1.00],[117.5,-0.26],[118.5,-1.28],[119.5,-1.80],[120.5,-3.34],[121.5,-2.08],[122.5,-1.02],[123.5,-1.54],[124.5,-1.54],[125.5,-1.54],[126.5,-1.00],[127.5,-1.50],[128.5,-0.16],[129.5,1.30],[130.5,2.00],[131.5,2.00],[132.5,1.44],[133.5,1.78],[134.5,0.70],[135.5,-0.12],[136.5,-1.72],[137.5,-1.10],[138.5,-2.08],[139.5,-1.92],[140.5,-1.28],[141.5,0.18],[142.5,-0.06],[143.5,-0.68],[144.5,-0.14],[145.5,-0.58],[146.5,-0.44],[147.5,0.72],[148.5,0.78],[149.5,-0.30],[150.5,0.96],[151.5,0.62],[152.5,-1.12],[153.5,-0.62],[154.5,0.44],[155.5,-0.82],[156.5,-2.16],[157.5,-1.96],[158.5,-1.46],[159.5,-1.06],[160.5,-1.00],[161.5,0.68],[162.5,2.28],[163.5,1.22],[164.5,-0.24],[165.5,0.76],[166.5,0.42],[167.5,-1.18],[168.5,0.42],[169.5,1.48],[170.5,0.34],[171.5,-1.00],[172.5,0.60],[173.5,-1.00],[174.5,-1.62],[175.5,-0.28],[176.5,0.34],[177.5,-0.70],[178.5,-0.16],[179.5,-0.60],[180.5,-0.80],[181.5,-0.28],[182.5,0.62],[183.5,0.08],[184.5,1.54],[185.5,0.54],[186.5,-0.52],[187.5,-0.52],[188.5,0.04],[189.5,-1.42],[190.5,-0.94],[191.5,-0.94],[192.5,-2.48],[193.5,-1.84],[194.5,-2.04],[195.5,-1.12],[196.5,-0.56],[197.5,0.06],[198.5,-1.14],[199.5,0.52],[200.5,0.12],[201.5,-0.64],[202.5,0.36],[203.5,0.28],[204.5,0.36],[205.5,-0.90],[206.5,0.76],[207.5,-0.78],[208.5,0.36],[209.5,-0.10],[210.5,1.16],[211.5,0.08],[212.5,1.68],[213.5,0.68],[214.5,1.20],[215.5,0.14],[216.5,-0.32],[217.5,-1.48],[218.5,-0.92],[219.5,-0.92],[220.5,-0.92],[221.5,-0.92],[222.5,-0.92],[223.5,-1.54],[224.5,-2.58],[225.5,-0.98],[226.5,0.56],[227.5,0.18],[228.5,0.62],[229.5,1.12],[230.5,-0.48],[231.5,-1.32],[232.5,-0.30],[233.5,0.86],[234.5,0.24],[235.5,1.70],[236.5,1.08],[237.5,0.44],[238.5,-1.16],[239.5,-0.00],[240.5,-1.46],[241.5,-0.40],[242.5,0.24],[243.5,0.78],[244.5,-0.20],[245.5,1.06],[246.5,1.60],[247.5,1.06],[248.5,1.14],[249.5,1.14],[250.5,0.50],[251.5,-0.02],[252.5,-0.56],[253.5,-0.56],[254.5,-1.18],[255.5,-0.72],[256.5,-1.18],[257.5,-0.12],[258.5,-0.82],[259.5,-0.82],[260.5,-1.98],[261.5,-2.16],[262.5,-2.14],[263.5,-1.00],[264.5,-1.00],[265.5,-0.38],[266.5,0.72],[267.5,1.10],[268.5,0.56],[269.5,0.62],[270.5,1.54],[271.5,-0.08],[272.5,-1.54],[273.5,-1.68],[274.5,-1.74],[275.5,-2.66],[276.5,-2.58],[277.5,-2.58],[278.5,-3.04],[279.5,-1.50],[280.5,-0.44],[281.5,1.10],[282.5,2.64],[283.5,3.78],[284.5,2.30],[285.5,0.70],[286.5,0.22],[287.5,-1.26],[288.5,-0.72],[289.5,-0.78],[290.5,-0.86],[291.5,-1.36],[292.5,-1.04],[293.5,-1.04],[294.5,0.42],[295.5,0.94],[296.5,0.38],[297.5,0.62],[298.5,0.28],[299.5,0.28],[300.5,0.38],[301.5,1.58],[302.5,2.42],[303.5,1.54],[304.5,1.16],[305.5,1.24],[306.5,0.04],[307.5,-1.62],[308.5,-0.54],[309.5,-1.70],[310.5,-2.32],[311.5,-1.26],[312.5,0.54],[313.5,-0.30],[314.5,-0.22],[315.5,-0.22],[316.5,-0.84],[317.5,-1.36],[318.5,-1.44],[319.5,0.02],[320.5,0.64],[321.5,1.80],[322.5,1.28],[323.5,1.28],[324.5,0.26],[325.5,0.18],[326.5,-0.80],[327.5,-1.30],[328.5,-1.28],[329.5,-0.66],[330.5,-0.14],[331.5,0.70],[332.5,1.08],[333.5,0.90],[334.5,-0.16],[335.5,0.32],[336.5,-1.14],[337.5,-1.02],[338.5,-1.48],[339.5,-1.04],[340.5,-1.96],[341.5,-0.70],[342.5,-1.46],[343.5,0.16],[344.5,-0.28],[345.5,-0.90],[346.5,-0.90],[347.5,-0.70],[348.5,-0.78],[349.5,-0.78],[350.5,-0.78],[351.5,-1.66],[352.5,-1.66],[353.5,-3.12],[354.5,-2.50],[355.5,-1.96],[356.5,-0.80],[357.5,-0.80],[358.5,-0.88],[359.5,-0.44],[360.5,0.62],[361.5,-0.38],[362.5,0.24],[363.5,1.52],[364.5,1.08],[365.5,1.02],[366.5,1.04],[367.5,0.42],[368.5,-0.24],[369.5,0.40],[370.5,0.40],[371.5,1.10],[372.5,2.36],[373.5,1.62],[374.5,0.36],[375.5,0.42],[376.5,-0.46],[377.5,-1.72],[378.5,-1.52],[379.5,-0.06],[380.5,-1.66],[381.5,-1.50],[382.5,0.10],[383.5,0.48],[384.5,-0.98],[385.5,0.10],[386.5,1.16],[387.5,-0.64],[388.5,0.24],[389.5,0.24],[390.5,-0.30],[391.5,-0.30],[392.5,0.34],[393.5,0.16],[394.5,0.08],[395.5,0.60],[396.5,-1.00],[397.5,0.02],[398.5,-1.06],[399.5,-1.06],[400.5,-1.06],[401.5,0.20],[402.5,-1.26],[403.5,-0.64],[404.5,1.04],[405.5,1.24],[406.5,-0.02],[407.5,1.58],[408.5,0.96],[409.5,-0.72],[410.5,-1.44],[411.5,-0.90],[412.5,-0.96],[413.5,-0.42],[414.5,-0.34],[415.5,0.66],[416.5,1.66],[417.5,0.56],[418.5,0.02],[419.5,0.58],[420.5,-0.34]];charge_datapoints = [[2.5,0.20],[3.5,0.00],[4.5,-0.20],[5.5,-0.20],[6.5,-0.40],[7.5,-0.20],[8.5,-0.20],[9.5,0.20],[10.5,0.30],[11.5,0.30],[12.5,0.10],[13.5,0.30],[14.5,0.30],[15.5,0.20],[16.5,0.20],[17.5,0.30],[18.5,0.30],[19.5,-0.10],[20.5,-0.30],[21.5,-0.30],[22.5,-0.40],[23.5,-0.60],[24.5,-0.60],[25.5,-0.40],[26.5,-0.20],[27.5,-0.20],[28.5,-0.00],[29.5,0.40],[30.5,0.60],[31.5,0.40],[32.5,0.40],[33.5,0.40],[34.5,-0.00],[35.5,-0.20],[36.5,-0.00],[37.5,-0.00],[38.5,-0.00],[39.5,0.20],[40.5,0.20],[41.5,-0.00],[42.5,-0.20],[43.5,-0.20],[44.5,-0.20],[45.5,-0.10],[46.5,-0.10],[47.5,0.10],[48.5,0.30],[49.5,0.10],[50.5,-0.00],[51.5,-0.00],[52.5,-0.00],[53.5,-0.20],[54.5,0.20],[55.5,0.30],[56.5,0.30],[57.5,0.30],[58.5,0.30],[59.5,0.30],[60.5,0.20],[61.5,0.20],[62.5,0.20],[63.5,-0.00],[64.5,-0.40],[65.5,-0.40],[66.5,-0.40],[67.5,-0.40],[68.5,-0.20],[69.5,-0.00],[70.5,-0.00],[71.5,-0.00],[72.5,-0.00],[73.5,-0.20],[74.5,-0.20],[75.5,-0.20],[76.5,-0.20],[77.5,-0.20],[78.5,-0.20],[79.5,-0.20],[80.5,-0.20],[81.5,-0.20],[82.5,-0.20],[83.5,-0.00],[84.5,-0.00],[85.5,-0.00],[86.5,-0.00],[87.5,0.10],[88.5,0.10],[89.5,0.10],[90.5,0.10],[91.5,0.10],[92.5,-0.20],[93.5,-0.40],[94.5,-0.60],[95.5,-0.60],[96.5,-0.60],[97.5,-0.20],[98.5,-0.00],[99.5,0.20],[100.5,0.40],[101.5,0.50],[102.5,0.30],[103.5,0.30],[104.5,0.30],[105.5,0.10],[106.5,-0.20],[107.5,-0.40],[108.5,-0.40],[109.5,-0.20],[110.5,-0.20],[111.5,0.00],[112.5,0.20],[113.5,0.20],[114.5,0.00],[115.5,0.00],[116.5,0.20],[117.5,0.00],[118.5,0.00],[119.5,0.00],[120.5,0.20],[121.5,0.00],[122.5,0.20],[123.5,0.40],[124.5,0.20],[125.5,0.20],[126.5,0.20],[127.5,0.20],[128.5,0.00],[129.5,0.20],[130.5,0.00],[131.5,0.00],[132.5,-0.20],[133.5,-0.20],[134.5,-0.20],[135.5,0.00],[136.5,0.00],[137.5,0.20],[138.5,0.20],[139.5,0.20],[140.5,0.00],[141.5,0.00],[142.5,0.00],[143.5,0.00],[144.5,0.00],[145.5,-0.20],[146.5,-0.20],[147.5,-0.20],[148.5,-0.10],[149.5,-0.30],[150.5,-0.10],[151.5,-0.10],[152.5,0.10],[153.5,-0.00],[154.5,0.20],[155.5,-0.00],[156.5,0.20],[157.5,-0.20],[158.5,-0.20],[159.5,-0.20],[160.5,0.10],[161.5,-0.10],[162.5,0.10],[163.5,-0.10],[164.5,-0.30],[165.5,-0.40],[166.5,-0.40],[167.5,-0.40],[168.5,-0.20],[169.5,0.00],[170.5,0.20],[171.5,0.40],[172.5,0.40],[173.5,0.20],[174.5,0.20],[175.5,0.00],[176.5,-0.20],[177.5,-0.20],[178.5,0.00],[179.5,0.00],[180.5,0.00],[181.5,0.00],[182.5,0.00],[183.5,0.00],[184.5,0.00],[185.5,0.10],[186.5,-0.10],[187.5,-0.10],[188.5,-0.10],[189.5,-0.30],[190.5,-0.40],[191.5,-0.40],[192.5,-0.20],[193.5,-0.20],[194.5,0.20],[195.5,0.20],[196.5,0.40],[197.5,0.20],[198.5,0.20],[199.5,0.00],[200.5,0.00],[201.5,0.20],[202.5,0.20],[203.5,0.40],[204.5,0.40],[205.5,0.60],[206.5,0.40],[207.5,0.20],[208.5,0.00],[209.5,0.00],[210.5,-0.20],[211.5,-0.20],[212.5,0.00],[213.5,0.10],[214.5,0.10],[215.5,-0.10],[216.5,0.10],[217.5,0.10],[218.5,0.00],[219.5,0.00],[220.5,0.00],[221.5,0.00],[222.5,0.00],[223.5,-0.20],[224.5,-0.20],[225.5,0.00],[226.5,-0.20],[227.5,-0.10],[228.5,0.10],[229.5,0.10],[230.5,0.10],[231.5,0.10],[232.5,0.00],[233.5,0.00],[234.5,0.00],[235.5,0.00],[236.5,-0.20],[237.5,-0.20],[238.5,-0.20],[239.5,-0.20],[240.5,-0.20],[241.5,0.00],[242.5,0.00],[243.5,0.00],[244.5,0.00],[245.5,0.00],[246.5,0.00],[247.5,0.00],[248.5,0.00],[249.5,0.00],[250.5,0.00],[251.5,0.00],[252.5,0.00],[253.5,0.00],[254.5,-0.20],[255.5,-0.20],[256.5,-0.20],[257.5,-0.20],[258.5,0.00],[259.5,0.00],[260.5,0.20],[261.5,0.20],[262.5,0.20],[263.5,0.00],[264.5,0.00],[265.5,-0.20],[266.5,-0.20],[267.5,-0.20],[268.5,-0.20],[269.5,0.10],[270.5,0.10],[271.5,0.30],[272.5,0.30],[273.5,0.30],[274.5,0.20],[275.5,0.20],[276.5,-0.20],[277.5,-0.20],[278.5,0.00],[279.5,0.00],[280.5,0.00],[281.5,0.20],[282.5,0.20],[283.5,0.00],[284.5,0.10],[285.5,0.10],[286.5,0.10],[287.5,0.20],[288.5,0.20],[289.5,0.10],[290.5,0.30],[291.5,0.30],[292.5,0.20],[293.5,0.20],[294.5,0.20],[295.5,0.00],[296.5,-0.20],[297.5,-0.20],[298.5,-0.20],[299.5,-0.20],[300.5,-0.20],[301.5,0.00],[302.5,0.00],[303.5,0.00],[304.5,0.00],[305.5,0.00],[306.5,0.00],[307.5,0.20],[308.5,0.20],[309.5,0.40],[310.5,0.40],[311.5,0.40],[312.5,0.20],[313.5,0.20],[314.5,-0.20],[315.5,-0.40],[316.5,-0.40],[317.5,-0.40],[318.5,-0.40],[319.5,-0.20],[320.5,0.00],[321.5,0.00],[322.5,0.00],[323.5,0.00],[324.5,0.00],[325.5,0.00],[326.5,0.00],[327.5,0.10],[328.5,0.10],[329.5,0.10],[330.5,0.10],[331.5,0.10],[332.5,0.00],[333.5,0.00],[334.5,-0.20],[335.5,-0.20],[336.5,-0.40],[337.5,-0.40],[338.5,-0.20],[339.5,0.00],[340.5,0.00],[341.5,0.20],[342.5,0.40],[343.5,0.20],[344.5,0.00],[345.5,0.00],[346.5,0.00],[347.5,-0.20],[348.5,-0.20],[349.5,0.00],[350.5,-0.20],[351.5,-0.20],[352.5,-0.20],[353.5,-0.40],[354.5,-0.40],[355.5,-0.20],[356.5,-0.20],[357.5,-0.40],[358.5,0.00],[359.5,0.00],[360.5,0.00],[361.5,0.00],[362.5,0.20],[363.5,0.00],[364.5,0.00],[365.5,0.00],[366.5,0.00],[367.5,0.00],[368.5,0.00],[369.5,0.00],[370.5,0.00],[371.5,0.00],[372.5,0.00],[373.5,0.20],[374.5,0.20],[375.5,0.20],[376.5,0.20],[377.5,0.00],[378.5,-0.40],[379.5,-0.40],[380.5,-0.40],[381.5,-0.40],[382.5,-0.20],[383.5,0.00],[384.5,0.00],[385.5,0.00],[386.5,0.00],[387.5,0.20],[388.5,0.20],[389.5,0.00],[390.5,0.00],[391.5,0.00],[392.5,-0.20],[393.5,-0.20],[394.5,0.20],[395.5,0.20],[396.5,0.00],[397.5,0.00],[398.5,-0.20],[399.5,-0.20],[400.5,-0.20],[401.5,0.00],[402.5,-0.20],[403.5,0.00],[404.5,-0.20],[405.5,-0.20],[406.5,-0.20],[407.5,0.00],[408.5,-0.20],[409.5,0.00],[410.5,0.00],[411.5,0.00],[412.5,0.00],[413.5,0.20],[414.5,-0.20],[415.5,-0.20],[416.5,-0.20],[417.5,-0.20],[418.5,-0.40],[419.5,-0.20],[420.5,-0.20]];dis_datapoints = [];trans_datapoints = [];sec_helix_datapoints = [[3,12],[28,34],[50,67],[81,90],[95,105],[109,121],[144,156],[159,172],[181,188],[193,200],[201,212],[215,218],[224,249],[261,276],[305,314],[359,365],[380,382],[389,391],[401,403]];sec_strand_datapoints = [[39,43],[72,76],[128,134],[282,286],[319,325],[330,334],[371,374],[406,411]];acc_exposed_datapoints = [[1,4],[7,7],[10,11],[15,15],[17,17],[19,19],[21,24],[26,26],[29,29],[33,33],[36,37],[66,67],[90,90],[92,92],[95,96],[99,100],[109,109],[120,120],[123,127],[141,141],[155,156],[158,160],[163,163],[167,167],[170,170],[173,174],[176,177],[179,179],[189,189],[191,193],[197,197],[200,200],[204,204],[207,207],[210,211],[214,214],[218,219],[221,221],[223,224],[251,253],[274,274],[277,279],[313,313],[316,317],[337,341],[343,343],[345,345],[347,347],[350,350],[353,353],[356,356],[364,365],[367,367],[369,369],[380,381],[384,384],[387,387],[397,397],[399,399],[402,402],[412,412],[414,414],[417,417],[420,423]];acc_buried_datapoints = [[5,5],[9,9],[13,13],[16,16],[28,28],[31,31],[35,35],[38,38],[40,50],[53,54],[56,61],[64,65],[69,79],[82,82],[84,86],[88,89],[91,91],[98,98],[101,102],[105,108],[111,115],[117,118],[122,122],[129,137],[142,143],[146,147],[149,151],[153,154],[161,162],[165,165],[168,169],[172,172],[183,187],[198,199],[201,202],[205,205],[209,209],[212,213],[216,217],[225,225],[228,232],[234,238],[240,242],[244,249],[258,258],[260,260],[262,262],[264,266],[268,270],[272,273],[280,280],[282,292],[294,294],[296,296],[298,298],[301,301],[304,311],[315,315],[319,327],[329,334],[346,346],[351,351],[358,359],[362,363],[366,366],[371,375],[378,379],[382,382],[388,388],[391,396],[400,400],[404,410],[415,415]];flot_plot_options = []; flot_plot_options[0] = {grid: {borderWidth: {top: 0,right: 0,bottom: 0,left: 0}},legend: {show: false},xaxes: [{show: true,min: 0,max: 200,ticks: [[0.5, '1'], [24.5, '25'], [49.5, '50'], [74.5, '75'], [99.5, '100'], [124.5, '125'], [149.5, '150'], [174.5, '175'], [199.5, '200']],tickLength: -5}],yaxes: [{show: true,ticks: [[0, '0'], [4.5,'hydro-
phobic  '], [-4.5,'hydro-
philic  ']],min: -4.5,max: +4.5,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(100,149,237,1)'}},{show: true,ticks: [[0, ], [1,'positive
 charge'], [-1,'negative
 charge']],position: 'right',min: -1,max: 1,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(255,99,71,1)'}}]};number_of_plots = 3;for ( plot_num = 1 ; plot_num < number_of_plots ; plot_num ++){flot_plot_options[plot_num] = $.extend(true, {} ,flot_plot_options[0]);flot_plot_options[plot_num].xaxes = [{min: plot_num*200,max: (plot_num + 1)*200,ticks: [ [plot_num*200 + 0.5, (plot_num*200 + 1).toString()], [plot_num*200 + 24.5, (plot_num*200 + 25).toString()], [plot_num*200 + 49.5, (plot_num*200 + 50).toString()], [plot_num*200 + 74.5, (plot_num*200 + 75).toString()], [plot_num*200 + 99.5, (plot_num*200 + 100).toString()], [plot_num*200 + 124.5, (plot_num*200 + 125).toString()], [plot_num*200 + 149.5, (plot_num*200 + 150).toString()], [plot_num*200 + 174.5, (plot_num*200 + 175).toString()], [plot_num*200 + 199.5, (plot_num*200 + 200).toString()] ],tickLength: -5}];};try {if( $('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_button').val() =='Show' ){$('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_container').css('display','block');$('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_button').val('Hide');var description_html = '
';description_html = description_html + '
 <input type=\'checkbox\' id=\'hydrophobicity_checkbox\' checked=\'checked\'> Moving average over 5 amino acids for hydrophobicity (<img src=\'https://static.igem.org/mediawiki/2013/e/e9/TUM13_hydrophobicity_icon.png\' alt=\'blue graph\' height=\'10\'></img>)';description_html = description_html + '
 <input type=\'checkbox\' id=\'charge_checkbox\' checked=\'checked\'> Moving average over 5 amino acids for charge (<img src=\'https://static.igem.org/mediawiki/2013/3/3e/TUM13_charge_icon.png\' alt=\'red graph\' height=\'10\'></img>)';description_html = description_html + '
 <input type=\'checkbox\' id=\'dis_checkbox\' checked=\'checked\'> Predicted disulfid bridges (<img src=\'https://static.igem.org/mediawiki/2013/2/28/TUM13_dis_icon.png\' alt=\'yellow circle\' height=\'10\'></img>) with the number of the bridge in the center';description_html = description_html + '
 <input type=\'checkbox\' id=\'trans_checkbox\' checked=\'checked\'> Predicted transmembrane helices (<img src=\'https://static.igem.org/mediawiki/2013/7/78/TUM13_trans_icon.png\' alt=\'turquois bars\' height=\'10\'></img>)';description_html = description_html + '
 <input type=\'checkbox\' id=\'sec_checkbox\' checked=\'checked\'> Predicted secondary structure: Helices (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_helix_icon.png\' alt=\'violet bars\' height=\'10\'></img>) and beta-strands (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_strand_icon.png\' alt=\'yellow bars\' height=\'10\'></img>)';description_html = description_html + '
 <input type=\'checkbox\' id=\'acc_checkbox\' checked=\'checked\'> Predicted solvent accessability: Exposed (<img src=\'https://static.igem.org/mediawiki/2013/1/16/TUM13_exposed_icon.png\' alt=\'blue bars\' height=\'10\'></img>) and buried (<img src=\'https://static.igem.org/mediawiki/2013/0/0b/TUM13_buried_icon.png\' alt=\'green bars\' height=\'10\'></img>) residues';description_html = description_html + '
';$('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_explanation').html(description_html);plot_according_to_selectors_1380912466041();$('#AutoAnnotator_container_1380912466041 #AutoAnnotator_plot_selectors').find('input').click(plot_according_to_selectors_1380912466041);}else{$('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_container').css('display','none');$('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_button').val('Show');$('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_explanation').html();}}catch(err){txt='There was an error with the button controlling the visibility of the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';alert(txt);}};function plot_according_to_selectors_1380912466041(){try{var plot_datasets = [[],[]];if($('#AutoAnnotator_container_1380912466041 #hydrophobicity_checkbox').prop('checked') == true){plot_datasets[0] = { color: 'rgba(100,149,237,1)',data: hydrophobicity_datapoints,label: 'Hydrophobicity',lines: { show: true, fill: true, fillColor: 'rgba(100,149,237,0.1)' },yaxis: 1};}if($('#AutoAnnotator_container_1380912466041 #charge_checkbox').prop('checked') == true){plot_datasets[1] = {color: 'rgba(255,99,71,1)',data: charge_datapoints,label: 'Charge',lines: { show: true, fill: true, fillColor: 'rgba(255,99,71,0.1)' },yaxis: 2};}for (plot_num = 0 ; plot_num < number_of_plots ; plot_num ++){$.plot('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_placeholder'+ plot_num.toString(), plot_datasets, flot_plot_options[plot_num] );}var screen_width = $('canvas.flot-base').width(); var pos_of_first_tick = 46;var pos_of_last_tick = screen_width - 51;var tick_diff = (screen_width - 97)/199;if($('#AutoAnnotator_container_1380912466041 #dis_checkbox').prop('checked') == true){for ( j = 0 ; j < dis_datapoints.length ; j++ ){$('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][0] - 1)/200) ).append('
' + (j+1) + '
');$('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][1] - 1)/200) ).append('
' + (j+1) + '
');}}if($('#AutoAnnotator_container_1380912466041 #trans_checkbox').prop('checked') == true){for ( j = 0 ; j < trans_datapoints.length ; j++ ){$('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_placeholder' + Math.floor((trans_datapoints[j][0] - 1)/200) ).append('
');}}if($('#AutoAnnotator_container_1380912466041 #sec_checkbox').prop('checked') == true){for ( j = 0 ; j < sec_helix_datapoints.length ; j++ ){$('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_placeholder' + Math.floor((sec_helix_datapoints[j][0] - 1)/200) ).append('
');}for ( j = 0 ; j < sec_strand_datapoints.length ; j++ ){$('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_placeholder' + Math.floor((sec_strand_datapoints[j][0] - 1)/200) ).append('
');}}if($('#AutoAnnotator_container_1380912466041 #acc_checkbox').prop('checked') == true){for ( j = 0 ; j < acc_buried_datapoints.length ; j++ ){$('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_placeholder' + Math.floor((acc_buried_datapoints[j][0] - 1)/200) ).append('
');}for ( j = 0 ; j < acc_exposed_datapoints.length ; j++ ){$('#AutoAnnotator_container_1380912466041 #hydrophobicity_charge_placeholder' + Math.floor((acc_exposed_datapoints[j][0] - 1)/200) ).append('
');}}}catch(err){txt='There was an error while drawing the selected elements for the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';throw(txt);}}</script></html>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 189
    Illegal XhoI site found at 599
    Illegal XhoI site found at 1189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316


[edit]
Categories
//cds/enzyme
//chassis/eukaryote/ppatens
Parameters
None