Part:BBa_K1155003

RBS-amilCP-term

AmilCP is a blue/purple chromoprotein from coral Acropora millepora. This protein exhibits a strong colour that we can visualize by naked eyes. The biobrick part (BBa_K592009) which including amilCP gene was created by Team Uppsala - Sweden 2011.

In order to be easily used by all the other teams, our team improved the biobrick BBa_K592009 by assembly of biobricks B0034, BBa_K592009 and B0015 to construct a composite part including a ribosome binding site, full length of amilCP gene and double terminator. We used it as one of our reporter gene in Escherichia coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization :

We have tested the Biobricks BBa_K1155003 in the construction: BBa_K1155000 + BBa_K1155003 in pSB1C3

BBa_K1155000 is a promoteur Pndh* repressed in anaerobic condition by binding of the FNR regulator.(see BBa_K1155000 for more information)

What we expected :

+ O2 : FNR inactivated : Pndh* activated --> amilCP expression --> purple color

- O2 : FNR activated : Pndh* repressed --> amilCP not expressed --> no color

What we observed :

+ O2 : --> purple color

- O2 : --> no color

These results indicate that the composite part BBa_K1155003 composed of a RBS + amilCP + terminator works very well and can be easily used as a reporter system of promoteur expression.