Coding

Part:BBa_K1075013

Designed by: Max Schelski   Group: iGEM13_Bonn   (2013-09-23)
Revision as of 02:12, 10 October 2013 by MaxSch (Talk | contribs)

AsLOV2-ipaA(L623A)

Fusion of LOV 2 (A. sativa) with the ipaA peptide designed by Lungu et al. in 2012. It forms light inducable a tight dimer with Vinculin.

Additional to their initial construct (BBa_K1075012) Lungu et al. further modified the system by mutations of the LOV-ipaA (L603A) construct and successfully weakend the baseline affinity for vinculin (initial design light state affinity to dark state affinity: 3.5 nM to 69 nM; mutant: 2.4 nM to >40µM affinity for vinculin) to reduce the dark state activity.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334866/]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None