Measurement
Part:BBa_K1055000:Experience
Designed by: Kai Fenzl Group: iGEM13_TU_Darmstadt (2013-09-18)
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Applications of BBa_K1055000
Characterization mKate
mKate2 is a red fluorescent protein that we want to use as a acceptor for FRET. According to Evrogen [2] mKate2 has an excitation maximum at 588 nm and an emission maximum at 633 nm. The emission maximum is big enough to get stimulated by LSSmOrange, the other fluorescent protein. Of course, we checked also the emission and excitation of our BL21(DE3) cells (data not shown) and we can eliminate the theories that these cells disturb this florescence measurement.
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