Coding

Part:BBa_K1104401:Design

Designed by: Jesse Wu   Group: iGEM13_NYMU-Taipei   (2013-10-04)
Revision as of 21:43, 4 October 2013 by Blackrabbit (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

pyruvate decarboxyase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1015
    Illegal AgeI site found at 1162
    Illegal AgeI site found at 1540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 49
    Illegal SapI site found at 1126


Design Notes

Source

PCR from Zymomonas mobilis using the following primers:

  • gcttctag atgagttatactgtcggtacctatttagcg
  • ggactgcagcggccgctactagta ctagaggagcttgttaacaggcttacg

References