Coding

Part:BBa_K1152001:Design

Designed by: Tania Christiansen   Group: iGEM13_Heidelberg   (2013-09-16)
Revision as of 18:22, 4 October 2013 by TaniaChristiansen (Talk | contribs) (→‎Design Notes)

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TycB1 Module from Tyrocidine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1195


Design Notes

This is just the coding sequence. Silent mutation was found after sequencing with VR and VF2 at positions 4595 (GCC to GCG; Alanin to Alanin) and at position 4598 (CTT to CTG; Leucine to Leucine)(http://2013.igem.org/File:Heidelberg_RFC10_TycASeq.zip and http://2013.igem.org/Team:Heidelberg/Tyrocidine_week20_biobrickmod#Sequencing ).

You can use it to combine it with other modules of e.g. the Tyrocidine cluster for the construction of a new peptide.

Go to our wiki: http://2013.igem.org/Team:Heidelberg/Parts

Source

Tyrocidine Gene Cluster of Brevibacillus Parabrevis. Genebank File taken from http://www.ncbi.nlm.nih.gov/nuccore/af004835

References