Part:BBa_K1123001
Protamine-1-Optimized Protein Production Construct
This composite part has been built up from a number of separate BioBricks, the most prominent of which the FNR promotor and the Protamine-1-Optimized Protein sequence. The FNR promotor sequence enables us to bring the Protamine-1-Optimized protein to expression anaerobically. Almost all E.coli strains posses a form of this promotor to induce metabolic processes once the bacteria enters anaerobic regions. The Protamine-1-Optimized protein is a human protein which we are expressing with the sole purpose of using it as a CEST contrast agent.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 200
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 39
Illegal XhoI site found at 359 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
This part is a composite part which is comprised of a number of other important parts namely BBa_K1123000 and BBa_K1123013.
This part has, in itself not been tested by us here at the TU-Eindhoven during the 2013 iGEM competition. However, as the other, important parts which make up this part have been tested, shown to work, and characterized we would like o refer you to these parts for a complete overview of the characterization of this part.
We do not think there will be a change in the functionality of any of the separate parts when combining them. A similar composite part BBa_K1123005 was combined and tested and showed no change in functionality other than a decrease in expression.
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