Device

Part:BBa_K1150022

Designed by: M. Stammnitz   Group: iGEM13_Freiburg   (2013-09-17)
Revision as of 17:37, 4 October 2013 by NightworkMax (Talk | contribs)

uniCAS Repressor (CMV promoter)

CMV:HA-NLS-dCas9-Linker-KRAB-NLS:BGH
Function Transcriptional Repression
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Organism Streptococcus pyogenes, Homo sapiens
Source Feng Zhang, Addgene
Konrad Müller, University of Freiburg
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

File:Https://static.igem.org/mediawiki/parts/a/a8/Freiburg2013 Plasmid Cas9-KRAB-1.jpg
Figure 1: Schematic overview of dCas9-KRAB composite part with all features.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BglII site found at 900
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5020
    Illegal SapI.rc site found at 4984


[edit]
Categories
Parameters
None