RBS
Part:BBa_K1045010:Design
Designed by: iGEM Team Göttingen 2013 Group: iGEM13_Goettingen (2013-09-20)
RBS BBa_B0034 with inversed Pre- and Suffix
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For cloning of the inverse RBS BBa_K1045010, hybridization oligos were used. Prefix and Suffix were switched to yield an inverted RBS.
Source
The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence originated from the Parts Registry page of BBa_B0034.