Coding

Part:BBa_K1123019

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-08-31)
Revision as of 22:58, 4 October 2013 by ArdjanvanderLinden (Talk | contribs) (→‎Characterization)

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Poly(Arginine-Serine) Protein

This part contains the DNA sequence of a protein of our own design. We first repeated the Arginine-Serine amino acid pair 6 times to obtain a sequence of 12 amino acids. This sequence was then repeated a total of 36 times. Between these repeats no extra amino acids were added. The idea behind this protein was to provide ourselves with a high concentration of amino acids with amide groups which we could then use to provide CEST contrast in an MRI. The protein can be placed behind a promoter of your choice and expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

For the iGEM 2013 Purdue team we characterized our parts according to their characterization datasheets. The data sheet for this particular biobrick can be found here.

Characterization by the 2013 TU-Eindhoven iGEM team

This part was created for use in the 2013 TU-Eindhoven iGEM project. Unfortunately however we did not get round to completing a proper characterization for this part as it went largely unused.


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Categories
Parameters
None