Coding

Part:BBa_K1067006

Designed by: Kristian Davidsen, Helen Cook and Jakob Berg Jespersen   Group: iGEM13_DTU-Denmark   (2013-09-24)
Revision as of 13:43, 12 October 2013 by Helencook (Talk | contribs)

Denitrification operon from Pseudomonas aeruginosa

This biobrick is the whole denitrification region from Pseudomonas aeruginosa PAO1 excluding only the enzyme in last step; Nitrous-oxide reductase ([http://ecocyc.org/PAER941193/NEW-IMAGE?type=LOCUS-POSITION&object=GLIQ-518&chromosome=CHROMOSOME-1 link to Biocyc]).


Usage and Biology

This brick has been shown hard to work with because it is a large region, all under one promoter. We purpose that splitting it into the 3 operons; like the conformation natively in P. aeruginosa and adding a separate promoter for each would make it easier to work with. If trying to to control the whole region under the same promoter one might experience premature mRNA transcripts because RNAP will fall of before the end of the region.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3445
    Illegal PstI site found at 6521
    Illegal PstI site found at 7162
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3445
    Illegal PstI site found at 6521
    Illegal PstI site found at 7162
    Illegal NotI site found at 4536
    Illegal NotI site found at 6121
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3445
    Illegal BglII site found at 197
    Illegal BglII site found at 2942
    Illegal BglII site found at 3335
    Illegal XhoI site found at 6639
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3445
    Illegal PstI site found at 6521
    Illegal PstI site found at 7162
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3445
    Illegal PstI site found at 6521
    Illegal PstI site found at 7162
    Illegal NgoMIV site found at 799
    Illegal NgoMIV site found at 1926
    Illegal NgoMIV site found at 1935
    Illegal NgoMIV site found at 2357
    Illegal NgoMIV site found at 2381
    Illegal NgoMIV site found at 2467
    Illegal NgoMIV site found at 3292
    Illegal AgeI site found at 1663
    Illegal AgeI site found at 6506
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 841
    Illegal BsaI.rc site found at 973
    Illegal BsaI.rc site found at 1706
    Illegal BsaI.rc site found at 3391
    Illegal BsaI.rc site found at 3693
    Illegal BsaI.rc site found at 5125
    Illegal BsaI.rc site found at 5676
    Illegal SapI site found at 6182
    Illegal SapI site found at 7434


[edit]
Categories
//cds/biosynthesis
//function/biosynthesis
Parameters
proteins