Part:BBa_K1088027:Experience

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UNIQf527517fe09bb6b5-partinfo-00000000-QINU UNIQf527517fe09bb6b5-partinfo-00000001-QINU

••• Patrick Rosendahl Andreassen

We investigated MG1655 strain carrying both BBa_K1088016 on pSB1K3 and BBa_K1088027 (CPS) by phase contrast microscopy and flow cytometry in order to evaluate whether there was a change in size or morphology compared to the wildtype (WT). As indicated by the forward side scatter measurements, there was a difference in size between CPS and MG1655 wildtype both before and after induction in late exponential phase (0.2% arabinose, 1 mM IPTG, 1 mM MgCl2) . Although both strains became small upon induction,the difference in size relation between the CPS and the wildtype remained. The difference in size was less clear during phase contract microscopy.

••• Patrick Rosendahl Andreassen

Growth curve of dxs devices. Cells were started at OD600=0.005 and incubated at 37oC and 180 rpm. 2 triplicates of MG1655 carrying no vector (WT), empty pSB1C3 vector (pSB1C3), pSB1C3-Plac-dxs(B.subtilis) (BBa_K1088011) (No LacI), pSB1C3-Pcon-lacI-Plac-dxs(B.subtilis) (BBa_K1088027) (LacI(N)), or pSB1C3-Pcon-lacI:LVA-Plac-dxs(B.subtilis) (BBa_K1088013) (LacI(LVA)). At time 2.5 hours one of each triplicate was induced with 1 mM IPTG. No growth change was observed.