Device

Part:BBa_K1028001

Designed by: Joseph Beton   Group: iGEM13_Leeds   (2013-09-18)
Revision as of 12:37, 4 October 2013 by Joebeton (Talk | contribs) (→‎Usage and Experimental Data)

INP.Si4 Silica Binding Complex

Introduction

http://i276.photobucket.com/albums/kk29/JosephBeetBeet/ff81cf4b-7969-4340-9a16-24e76dc3e5e8.jpg
This biobrick was designed utilise Ice Nucleation Protein to express the Si4 Binding Peptide on the extracellular surface of the plasma membrane of E.coli cells.

Usage and Experimental Data

Fig.1: Monitoring the growth rate of cells containing Si4 Binding Peptide attached to the C-terminus of INP, labelled here as Device 2, within the PSB1C3 standard iGEM submission plasmid. Although the graph shows a log and a lag phase for the cells containing the insert plasmid, the absorbance reached in log phase is very low compared to the control cells.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 409
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None