Part:BBa_K1028000
Silica binding peptide
Introduction
This BioBrick is a short peptide of 15 amino acids designed to act as a tag binding with high affinity to silica and silica-like particles. As a tag it can easily be added to the C-terminus of proteins using molecular biology techniques allowing the peptide to assist with purification or to serve other experimental processes related to silica binding.
Figure.1 Illustrated schematic of the Si4 Binding Peptide binding with a silica microsphere.
This part was originally designed by the Leeds 2013 iGEM team to bind with silica microspheres, designed to act as pathogen surrogates, when presented on the exterior of the plasma membrane via fusion with Ice Nucleation Protein.
Usage and Experimental Data
Fig.1: Monitoring the growth rate of cells containing Si4 Binding Peptide within the PSB1C3 standard iGEM submission plasmid. Although the graph shows a log and a lag phase for the cells containing the insert plasmid, the absorbance reached in log phase is very low compared to the control cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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