Part:BBa_K1150025
uniCAS Red Light Switch Part I - Stimulator
uniCAS red Light Switch Part I - Stimulator | |
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Function | dCas9-protein linked to PIF6
induced gene expression control |
Use in | Mammalians |
RFC standard | RFC 25 |
Backbone | pSB1C3 |
Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Introduction
Our construct contains the standardized version of dCas9 linked to PIF6. This device is one of two parts that you need to enable red-light induced gene targeting. Cotransfection of a PhyB+effector device, and target RNA allows you to recruit any desired effector to your desired gene locus. Illumination with red light (660nm) will start the interaction and illumination with far red light (740nm) will stop the interaction. The Cas9-version used here is human codon optimized and the mutated dCas9-version is unable to cleave DNA. PIF6 is human codon-optimized. Have a look at our [http://2013.igem.org/Team:Freiburg/Project/induction#light light project page].
References:
Muller, K.; Engesser, R.; Metzger, S.; Schulz, S.; Kampf, M. M.; Busacker, M. et al. ((Nucleid Acid Research; 2013): A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells.
n/a | uniCAS Red Light Switch Part I - Stimulator |