Composite

Part:BBa_K1065303:Design

Designed by: Fabio Digiacomo   Group: iGEM13_UNITN-Trento   (2013-08-13)
Revision as of 20:42, 3 October 2013 by Cridelbianco (Talk | contribs) (→‎Source)

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B0034-YF1-B0034-FixJ-pFixK2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 634
    Illegal NgoMIV site found at 724
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 1547
    Illegal NgoMIV site found at 1641
    Illegal AgeI site found at 276
    Illegal AgeI site found at 1422
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1311
    Illegal BsaI.rc site found at 175


Design Notes

This part has been made following the standard assembly, by inserting BBa_K592006 inside BBa_K592016

Source

YF1 is a fusion protein of YtvA (from B subtilis ) and FixL (from B japonicum); FixJ and pFixK2 come from the genome of B. japonicum. The part consists of:
BBa_K592016
BBa_K592006

References

1) Moglich A, Ayers RA and Moffat K. (2009) Design and Signaling Mechanism of Light-Regulated Histidine Kinases. J. Mol. Bio. 385, 5, 1433-1444.
2)Ohlendorf, R., Vidavski, R.R., Eldar, A., Moffat, K. & Möglich, A.(2012). From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. J. Mol. Biol., 416: 534: 542