Composite

Part:BBa_K1075028

Designed by: Marc Schulte   Group: iGEM13_Bonn   (2013-09-23)
Revision as of 16:04, 3 October 2013 by AnnikaS (Talk | contribs)

No part name specified with partinfo tag.


construction

The part contains the promoter plac, a ribosomal binding site, the red fluorescent protein mCherry and a double terminator. It is integrated in the plasmid pJD427, which contains a SspB split system.


biology

The plasmid pJD427 contains the two parts of splitted SspB, which can be fused (and thus regain function) by addition of rapamycin. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220803/

plac is a IPTG inducible promoter.

The ribosomal binding side is a sequence of mRNA where the ribosome binds to start translation.

mCherry is a red fluorescent protein with the excitation maximum at 587 nm and the emission maximum at 610 nm. http://www.ncbi.nlm.nih.gov/pubmed/15558047

The double terminator stops the transcription at this point.

application

This plasmid is a negative control to the pJD427-pLac2-RBS34-mCherry-ssrA-TT plasmid. [[1]]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 102
    Illegal BsaI.rc site found at 743


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