Part:BBa_K1022114
pT7 : Lysis Device
This bio-brick codes for the promoter pT7(BBa_I712074) followed by the lysis device(BBa_K112808).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1404
Illegal NheI site found at 1427 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1009
Illegal AgeI site found at 1079 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1660
Characterization
For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch TU Delft iGEM13 Wiki !!]
The construct is made by ligating the pT7 promoter BBa_I712074 in front of the lysis device from the biobrick BBa_K112808. This is analyze how the lysis device can be controlled if needed.
Description : The E.coli cells transformed with pT7 lysis cassette BBa_K1022114 is grown on a plate reader which is capable of shaking and heating to 37˚C to take readings of the cells in exponential phase at every 10 minutes. Different range of IPTG concentration is used to characterize the bio – brick BBa_K1022114. At time point, 160 minutes, IPTG is added according to the table below.
No cells + 1mM | 0.1mM | 0.2m | 0.3mM | 0.4mM | 0.5mM | 0.6mM | 0.7mM | 0.8mM | 0.9mM | 1mM Cell + No IPTG | ||
---|---|---|---|---|---|---|---|---|---|---|---|---|
LB(µL) | 90 | 94 | 93 | 92 | 91 | 90 | 89 | 88 | 87 | 86 | 85 | 95 |
Cells(µL) | - | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
10X IPTG(µL) | 10 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | - |
n/a | pT7 : Lysis Device |