Coding
Part:BBa_K1197014:Design
Designed by: Alişan Kayabölen Group: iGEM13_METU_Turkey (2013-10-02)
House fly cytochrome P450 reductase (fCPR)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1247
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1769
Design Notes
It is used as natural. Biobrick prefix and suffix were added by primers.
Source
Its source is Musca domestica genome.
References
Andersen, J.F., Utermohlen, J.G., Feyereisen, R., 1994. Expression of house fly CYP6A1 and NADPH-cytochrome P450 reductase in Escherichia coli and reconstitution of an insecticide-metabolizing P450 system. Biochemistry 33, 2171e2177.