Coding

Part:BBa_K1197013:Design

Designed by: Alişan Kayabölen   Group: iGEM13_METU_Turkey   (2013-10-02)
Revision as of 02:15, 3 October 2013 by Alisankayabolen (Talk | contribs) (→‎Source)


CYP6G1 - Insecticide resistance gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 542
    Illegal XhoI site found at 1210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 943


Design Notes

Original sequence was modified since it was not proper for prokaryotic expression. Its first 21 nucleotide which coding 7 aa was changed with "Barnes" sequence: MVLTEVL is the native aa sequence, and MALLLAV is the Barnes aa sequence. (Barnes et al. 1991)

Reference: Barnes, H.J., Arlotto, M.P., Waterman, M.R., 1991. Expression and enzymatic activity of recombinant cytochrome P450 17a-hydroxylase in Escherichia coli. Proc. Natl. Acad. Sci. USA 88, 5597e5601.


Source

Its source is Drosophila melanogaster genome and it is modified according to work in B.subtilis.

References