Composite

Part:BBa_K1149004

Designed by: Margarita Kopniczky   Group: iGEM13_Imperial_College   (2013-09-13)
Revision as of 23:43, 2 October 2013 by Jps09 (Talk | contribs)

pelB-pueB expression

PUR degradation enzyme from Pseudomonas chlororaphis.

PUR_esterase_theoretical_pathway_2.jpg

Characterisation

Our PueB construct was tested for growth upon induction by addition of Xylose.

450px-PueB.png

PueB growth assay. E. coli (MG1655) transformed with PueB BBa_K1149004 were grown with either 0% or 2% Xylose to induce PueB expression. There was no difference between no induction and induction as a t-test gave p = 0.6040 > 0.05. Growth was at 37°C in LB with shaking. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM.

Conclusion: There is no growth inhibition caused by induction in PueB.

References:

[http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)]

[http://www.sciencedirect.com/science/article/pii/S0964830502000690 secretion and expression in E.coli]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BglII site found at 1213
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 407
    Illegal AgeI site found at 752
    Illegal AgeI site found at 1241
    Illegal AgeI site found at 1517
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None