Composite

Part:BBa_K1111009

Designed by: Luisa Spisak, Marcelle Isaline Arrigo   Group: iGEM13_EPF_Lausanne   (2013-09-19)
Revision as of 19:02, 1 October 2013 by Miarrigo (Talk | contribs)

Gelatinase under pBAD/araC arabinose inducible promoter

This construct is composed of gelatinase E's (gelE) coding sequence(CDS)inserted between the pBAD/araC promoter and superfolder GFP of part BBa_I746908 from Cambridge 2007. A linker is also present to join gelE and GFP correctly and to allow proper folding. Upon arabinose addition in the culture medium of bacteria transformed with this plasmid, gelE-GFP will be expressed and secreted. The main idea of this part was to allow easy replacement of the promoter driving the gelatin-degrading enzyme by another that responds to other specific and desired conditions. In our project, MMP2 would be secreted and would degrade nanoparticles attached to the bacterial surface and containing drugs, thus releasing the drugs only when arabinose is added to the culture medium.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3101
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal PstI site found at 3101
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2634
    Illegal BglII site found at 2685
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3028
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3101
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3101
    Illegal NgoMIV site found at 2330
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2703
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1517
    Illegal BsaI.rc site found at 2234
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 3169


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Categories
Parameters
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