Generator

Part:BBa_K1060013

Designed by: Ingmar Claes   Group: iGEM13_KU_Leuven   (2013-09-24)
Revision as of 09:53, 30 September 2013 by IngmarClaes (Talk | contribs)

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LacI-AroG* production under regulation of TetR repressible promoter

Regulatory construct to get expression of LacI (BBa_C0012) and mutated AroG (AroG*) (for details see the wild-type AroG; BBa_K1060000) under the control of the TetR repressible promoter (BBa_R0040). The AroG* (Pro150Leu and Leu175Asp) is less repressed by phenylalanine resulting in a upscaling of the shikimate pathway and production of more chorismic acid (ChA). ChA being a precursor of methyl salicylate. A biosensor which expresses TetR (BBa_C0040) under the certain conditions could switch off LacI and AroG* expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1228
    Illegal XhoI site found at 2195
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1502


[edit]
Categories
Parameters
None