Composite

Part:BBa_K1127002

Designed by: Jonas Kondratavicius   Group: iGEM13_York_UK   (2013-09-08)
Revision as of 23:55, 29 September 2013 by Kobachii.d (Talk | contribs) (→‎Characterization)

IM9 and A3-Flg fusion for gold binding

This is the inverted version of the part BBa_K1127020 with the same function.

Characterization

We tried to characterize the part using Nanoparticle Tracking Analyzer from NanoSight [http://www.nanosight.com]. The part was cloned into BBa_J23100 [1] which allows constitutive expression of the peptide.

We obtained recording (below) and several analytical reports of the particles within samples from the overnight cultures (E. coli strain DH5 alpha + BBa_K1127002/BBa_J23100 hybrid). The results can't provide evidence for gold bio-mineralization by the peptide because changes were subtle and not significant compared to the controls. Please visit our wiki page for more information [http://2013.igem.org/Team:York_UK].

File:MIDAS2 gold binding peptide.mp4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    COMPATIBLE WITH RFC[1000]


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