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Translational_Unit

Part:BBa_K1223011:Design

Designed by: Orr Schlesinger   Group: iGEM13_BGU_Israel   (2013-09-10)
Revision as of 13:49, 12 October 2013 by Alex m (Talk | contribs)

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ampR translational unit (ampicilin resistance CDS+promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 154


Design Notes

AmpR gene encodes for the beta-lactamase (EC 3.5.2.6) protein that confers resistance against beta-lactam antibiotics (ampicillin, carbanecillin).

this construct is widely used for selection of transformants. this part contains constitutive promoter, CDS and the stop codon and can be used as a whole to insert a selection marker into composite, larger biobricks or plasmids.

Source

part extracted from pKD4 plasmid by PCR.

References

http://en.wikipedia.org/wiki/Beta-lactamase