Generator

Part:BBa_K1060003:Experience

Designed by: Ingmar Claes, Robbert Boudewijns, Laurens De Backer   Group: iGEM13_KU_Leuven   (2013-09-17)
Revision as of 08:45, 4 October 2013 by LMarcelis (Talk | contribs) (→‎Applications of BBa_K1060003)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Characterization of BBa_K1060003

BBa_K1060003.jpg

E. coli BL21(DE3) strain

IPTG: 0,2mM

salicylate: 1mM

chorismate: 1mM

control: BL21(DE3) without plasmid and antbiotics

User Reviews

UNIQ7baa27b231b41c83-partinfo-00000000-QINU UNIQ7baa27b231b41c83-partinfo-00000001-QINU

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Sander Wuyts

The KU Leuven iGEM 2013 team tried to perform a qPCR on this part. It was impossible to remove the original plasmid DNA, after RNA isolation, even after several attempts with different DNase treatments. This problem is probably due to the fact that this part was provided in a high copy number backbone. If you want to perform a qPCR yourself, we recommend you to clone this part in another backbone or in the genome itself.

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