Device

Part:BBa_K1065101

Designed by: Caterina Marchioretti   Group: iGEM13_UNITN-Trento   (2013-06-25)
Revision as of 12:23, 29 September 2013 by Cate (Talk | contribs)

AraCpBAD + BSMT1

This is the part BBa_K1065100 under the control of the inducibile promoter AraCpBAD. This promoter allows to obtain the production of Methyl Salicylate starting from Salicyclic Acid when 5 mM of Arabinose was added. This part was cloned by the iGEM UniTN Trento 2013 team for the creation of Wintergreen Device BBa_K1065106.

Usage and Biology

BSMT1 catalyzes the conversion of salicylic acid to methyl salicylate, which has a wintergreen smell.

The arabinose inducible promoter BBa_K731201 was fully characterized by iGEM UniTN Trento 2012.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1893
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1434
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None