Composite

Part:BBa_K1065307:Design

Designed by: Caterina Marchioretti   Group: iGEM13_UNITN-Trento   (2013-09-15)
Revision as of 20:29, 28 September 2013 by Fabius924 (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


J23100+YF1+FixJ+terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


Design Notes

This part was built following the standard assembly protocol: BBa_K1065306 has been joined with BBa_B0015.

Source

This part has been built using:
BBa_K1065306: it produces YF1 and FixJ under the control of a strong promoter of the Anderson family.
BBa_B0015: the terminators.

References

1. Andreas Möglich, Rebecca A. Ayers, Keith Moffat (2009). Design and Signaling Mechanism of Light-Regulated Histidine Kinases. Journal of Molecular Biology, 1433–1444.
2) Ohlendorf, R., Vidavski, R.R., Eldar, A., Moffat, K. & Möglich, A.(2012). From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. J. Mol. Biol., 416: 534: 542