Reporter

Part:BBa_K1151036

Designed by: Gian Piero Lazzari   Group: iGEM13_UniSalento_Lecce   (2013-09-23)
Revision as of 16:01, 28 September 2013 by LeleBiotec (Talk | contribs) (→‎Fluorescence decay assay)

Double generator NikR-GFP, IPTG-nickel regulated

A simple construct composed of K1151006 + K1151009, used for the NikR activity study.

Fluorescence decay assay

Gfp1.jpg Gfp2.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 818
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 818
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 818
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 818
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 818
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1616


[edit]
Categories
Parameters
n/aDouble generator NikR-GFP, IPTG-nickel regulated