Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Coding

Part:BBa_K1189031

Designed by: Ali Honarmand, Somshukla Chaudhuri   Group: iGEM13_Calgary   (2013-09-17)
Revision as of 22:55, 29 September 2013 by Cmwinter (Talk | contribs)

TALE-A linked to beta-lactamase with a his tag under a lacI promoter

This part was built to function as both our detector (TALE A) and our reporter (beta-lactamase). The part was built with the lacI IPTG inducible promoter J04500, with RBS, and it has a His-tag for protein purification.

TALE A was inspired by the award winning <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K782004">TALE A</a> from the award winning <a href="http://2012.igem.org/Team:Slovenia">2012 Slovenian iGEM project</a>. The <a href="2013.igem.org/Team:Calgary">iGEM Calgary 2013</a> used this TALE and its associated DNA binding sequence to build a proof of concept TALE based DNA detector. In the case of <a href="https://parts.igem.org/Part:BBa_K1189031">BBa_K1189031</a>, the Calgary team used beta-lactamase as a reporter enzyme to indicate when the TALE is bound to DNA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1922
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2651
    Illegal AgeI site found at 677
    Illegal AgeI site found at 1258
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None