DNA

Part:BBa_K1129012

Designed by: UBC iGEM 2013   Group: iGEM13_British_Columbia   (2013-08-30)
Revision as of 00:10, 28 October 2013 by AnnaMüller (Talk | contribs)

T7 Repeat/Spacer Region of T7 Bacteriophage

T7 Repeat/Spacer Region of Streptococcus thermophilus


This part confers resistance against T7 phage upon a strain containing the Cas9 and TracrRNA parts.

This part is computationally verified to target all of 13a, K30, T3 and T7 genomes

Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] By associating with CAS9, an enzyme with both endonuclease and DNA-binding activity, CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner similar to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR Sequence and Features


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None