Generator
Part:BBa_K1189033:Design
Designed by: Ali Honarmand Group: iGEM13_Calgary (2013-09-27)
EHEC TALE2 (binds to a 19bp region of stx2 gene)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2404
Illegal BamHI site found at 2539 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A KasI restriction cut site has been incorporated in the end of the TALE, so that part can easily be used to replace the TALEs in our other constructs.
Source
This part is being constructed using IDT G-Blocks.
References
Meckler, J.F., Bhakta, M.S., Kim, M.S., Ovadia, R., Habrian, C.H., Zykovich, A., ... Baldwin, E.P. (2013). Quantitative analysis of TALE-DNA interactions suggests polarity effects. Nucleic Acids Research, 41(7), 4118–28. doi:10.1093/nar/gkt085