Part:BBa_K1092003:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Characterization of BBa_K1092003

1. Biobrick DNA length verification To check the inserted biobrick DNA length, PCR was conducted.

The PCR product meets the target size (936bp), the DNA sequence was further verified by DNA sequencing.

2. Time series degradation assay Catechol 1,2-dioxygenase is resposible for phenol degredation (Naiem et al., 2011).

Time series analysis were performed. According to a chi-square test (Rosenberg et al., 1998), the trends of hydroquinone degradation rate in E. coli BL21 transformed with catechol 1,2-dioxygenase and that in wild type E. coli BL21 were very highly statistically different (p < 0.001) (Figure 1).

User Reviews

UNIQ6c511f3c4cf59027-partinfo-00000000-QINU UNIQ6c511f3c4cf59027-partinfo-00000001-QINU