Measurement

Part:BBa_K1100011:Design

Designed by: YingQi Lou   Group: iGEM13_Fudan   (2013-09-13)
Revision as of 23:43, 27 September 2013 by Nzy (Talk | contribs) (→‎Design Notes)

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B0015 terminator efficiency estimation device (with Csy4 system)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 164


Design Notes

In this device, we test the terminator efficiency in the consideration that the terminator is influenced by the downstream sequence. The plasmid map of K1100011.png

Source

The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab. sfGFP from Ding Yu's Lab at Fudan University, R0040 from Kit, B0034 via PCR, Insulator: Csy4 loci via PCR, B0015 from Kit.

References

Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.