Generator

Part:BBa_K1124011

Designed by: Yuta Otsuka   Group: iGEM13_UT-Tokyo   (2013-09-11)
Revision as of 22:44, 27 September 2013 by Minakuchi.T (Talk | contribs) (→‎Usage and Biology)

hpaBC (+RBS(natural))

Usage and Biology

This part consists of hpaB and hpaC genes, and is called hpaBC The products of these genes, HpaB and HpaC, together form an enzyme 4-hydroxyphenylacetate 3-hydroxylase. This enzyme hydroxylates L-tyrosine to produce L-DOPA in vivo.

It is known that hpaBC overexpression leads to increase of L-DOPA production in E. coli. We designed L-DOPA synthesis device(Part:BBa_K1124107) by combining this part and tyrosine synthesis device(Part:BBa_K1124106).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 97
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1877


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Categories
Parameters
None