Part:BBa_K1100062

RNA-OUT S4

It is amongst the mutant library of RNA-OUT, part of a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. Earlier studies have suggested that the 5 base pairs in the 5’ end of RNA-IN and in the loop domain of RNA-OUT determine the initiation of RNA duplex formation. The mutation of these 5 base pairs resulted in a library of orthogonal RNA regulators of translation. The translation repression rate of these regulators and the cross-talk of them are shown as followed:

Figure 1. Repression rate

Figure 2. RNAIN/OUTS04 binding duplex

Figure 3. predicted structure of RNAOUTS4

References

Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]