DNA

Part:BBa_K1195000:Design

Designed by: Clarice Harrison   Group: iGEM13_BYU_Provo   (2013-08-28)
Revision as of 22:17, 27 September 2013 by Mschellhous (Talk | contribs)

Dispersin B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 970
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No specific design considerations were needed.


Source

iGem part BBa_K802001 received from the Lyon-INSA iGem team. We also amplified the gene from Actinobacilus Pleuropneumoniae.

References

Kaplan, J. Therapeutic Potential of Biofilm-Dispersing Enzymes. Int. J. Artif. Organs 2009, 32, 545-554.

Ragunath, C.; Shanmugam, M.; Bendaoud, M.; Kaplan, J.; Ramasubbu, N. Effect of a Biofilm-Degrading Enzyme from an Oral Pathogen in Transgenic Tobacco on the Pathogenicity of Pectobacterium Carotovorum Subsp Carotovorum. Plant Pathol. 2012, 61, 346-354.

Gokcen, A.; Vilcinskas, A.; Wiesner, J. Methods to Identify Enzymes that Degrade the Main Extracellular Polysaccharide Component of Staphylococcus epidermidis Biofilms. Virulence 2013, 4, 260-270.