Part:BBa_K1024003

Reporter for Tet system in Yeast

Part Name: BBa_K1024003

Short Description: Reporter for Tet system in Yeast

Part Type: Signaling

Design: The part is designed to valid the Tet system in yeast. TetR gene with VP16 is constitutively expressed, activating the downstream reporter gene of TetO and CYC1 TATA region. In the yeast Saccharomyces cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Thus, it could be used as a marker for screening of target phenotype.

Figure 1. The principle of Tet system

Test: Yeast with ADE2 knockout exhibits red in color. The function of the plasmid was tested by rescuing the knockout strains, which regained the white color by expressing ADE2.

Figure 2. Rescuing the ADE2 knockout yeasts

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 3077
  Illegal BamHI site found at 2480
  Illegal XhoI site found at 1779
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]