Part:BBa_J31000:Design

DNA-invertase Hin from Salmonella typhimurium

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is cloned into plasmid pSB1A2.

Biobricks

The Biobricks attached to this part are not wild type but the cut sites are still viable.

GAATTCGCGGCCGCTTCTAGA-ATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTACCAGTGCAAATTGTGACCGCATTTTTGAGGACCGTATCAGTGGCAAGATTGCAAACCGCCCCGGCCTGAAACGAGCGTTAA

AGTATGTAAATAAAGGCGATACTCTTGTCGTCTGGAAATTAGACAGACTGGGCCGCAGCGTGAAAAACCTGGTGGCGTTAATATCAGAATTACATGAACGTGGAGCTCACTTCCATTCTTTAACCGATAGTATTGATACCAGTAGCGCGATGGGGCGAT

TCTTTTTTCATGTAATGTCAGCACTGGCCGAGATGGAGCGAGAATTAATTGTCGAGCGAACCCTTGCCGGACTGGCTGCCGCCAGAGCGCAAGGACGACTGGGAGGGCGCCCTCGGGCGATCAACAAACATGAACAGGAACAGATTAGTCGGCTATTA

GAGAAAGGCCATCCTCGGCAGCAACTAGCTATTATTTTTGGTATTGGCGTATCTACCTTATACAGATATTTTCCGGCAAGCCGTATAAAAAAACGAATGAATTAA-ACTAGT -GCGGCCGCCTGCAG

There is no G spacer between the XbaI and the insert.

There is no T spacer between the insert and the SpeI site.

There is no A spacer between the SpeI site and the NotI site.

The last C of the NotI site is not conserved with the initial C from the PstI site.

The BB suffix currently has this sequence for NotI and PstI: GCGGCCGcCTGCAG

But it should have been: GCGGCCGCTGCAG

There is no spacer between the NotI site and the SpeI site. There should be a T.

We compared our BioBricks with those from Tom Knight's paper, Idempotent Vector Design for Standard Assembly of Biobricks. As seen below

Source

Salmonella typhimurium

References

Knight, Tom. Idempotent Vector Design for Standard Assembly of Biobricks