Part:BBa_K1166005

TAT-Apoptin

Codes for the T7 expression cassette for Apoptin composed of a N-terminal TAT translocation peptide, followed by a linker (GGGGS), a histidine tag (6x) and by the same linker fused to Apoptin. Apoptin, also called VP3, is a protein from the Chicken Anemia Virus (CAV) known to cause p53-independent apoptosis in more than 70 human cancer cell lines while leaving normal cells unharmed (Backendorf, et al., 2008).

Apoptin contains a bipartite nuclear localization signal (NLS1: aa 82-88 and NLS2: aa 111-121) and a nuclear export signal (NES) (aa 97-105) (Heckl, et al., 2008). In cancer cells, Apoptin localizes to the nucleus while in normal cells it's found in the cytoplasm; it is thought that the bipartite NLS is activated by phosphorylation at Threonine 108 which only happens in cancer cells.

References

Backendorf C, Visser AE, de Boer AG, Zimmerman R, Visser M, Voskamp P, Zhang YH, Noteborn M. (2008). Apoptin: therapeutic potential of an early sensor of carcinogenic transformation. Annu Rev Pharmacol Toxicol. 48:143-69.

Heckl S, Regenbogen M, Sturzu A, Gharabaghi A, Feil G, Beck A, Echner H, Nagele T. (2008). Value of apoptin's 40-amino-acid C-terminal fragment for the differentiation between human tumor and non-tumor cells.Apoptosis. 13(4):495-508

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]