Signalling

Part:BBa_K1024003:Design

Designed by: Sun Xiaochen   Group: iGEM13_Tsinghua   (2013-09-09)
Revision as of 17:04, 27 September 2013 by Sxc (Talk | contribs) (→‎Source)

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Reporter for Tet system in Yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3077
    Illegal BamHI site found at 2480
    Illegal XhoI site found at 1779
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design: The part is designed to valid the Tet system in yeast. TetR gene with VP16 is constitutively expressed, activating the downstream reporter gene of TetO and CYC1 TATA region. In the yeast Saccharomyces cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Thus, it could be used as a marker for screening of target phenotype.

Source

1.S. cerevisiae: ADE2
2. Registry: K079046, K1119006
3. Dai' Lab, Tsinghua University: CYC1 Terminator

References

Bellí G, Garí E, Piedrafita L, et al. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast[J]. Nucleic acids research, 1998, 26(4): 942-947.