Reporter

Part:BBa_K1024001:Design

Designed by: Sun Xiaochen   Group: iGEM13_Tsinghua   (2013-09-09)
Revision as of 16:19, 27 September 2013 by Fanxiao0606 (Talk | contribs) (→‎Design Notes)

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Lux Promoter in Yeast (pLux+Cyc Promoter+mCherry)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We constructed and improved standard BioBrick Parts about quorum sensing systems and modified the systems in prokaryotic microorganisms for usage in S. cerevisiae (Yeast). We modified the transcriptional regulated promoter in quorum sensing system, Plux promoter (BBa_R0062), by adding cyc100 mini promoter downstream of the Plux promoter (BBa_K1024001).

Source

S. cerevisiae & Registry

References


[1]Fuqua W C, Winans S C, Greenberg E P. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators[J]. Journal of bacteriology, 1994, 176(2): 269.
[2]Sadowski I, Ma J, Triezenberg S, et al. GAL4-VP16 is an unusually potent transcriptional activator[J]. Nature, 1988, 335(6190): 563-564.