Composite

Part:BBa_K1149052

Designed by: Margarita Kopniczky   Group: iGEM13_Imperial_College   (2013-09-26)
Revision as of 13:49, 27 September 2013 by Margarita K (Talk | contribs)

Constitutive phaCAB

This part has a strong E.coli constitutive promoter in front of the phaCAB PHB biosynthetic operon. Nile Red stain, under UV, shown PHB accumulated inside the cells:

27-9-13phaCABconstitutive.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 627
    Illegal BglII site found at 1452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 604
    Illegal NgoMIV site found at 916
    Illegal NgoMIV site found at 1195
    Illegal NgoMIV site found at 1847
    Illegal NgoMIV site found at 1869
    Illegal NgoMIV site found at 2491
    Illegal NgoMIV site found at 2632
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3713


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Categories
Parameters
None